| Name | Volume |
|---|---|
| DMEM (+ 4.5 g/L D-glucose, + L-Glut, − Sodium Pyruvate) | 250 mL |
| F-12 (+L-Glut) | 250 mL |
| FBS Note: Do not heat inactivate FBS. Just thaw, swirl to mix, and go. |
10.5 mL |
| Insulin-transferrin-selenium-X (100x) | 5.3 mL |
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