Key Resource Table:

REAGENT or RESOURCE	SOURCE	IDENTIFIER
4% paraformaldehyde	ThermoFisher	J61899-AK
Beta-Mercaptoethanol	Gibco	21985-023
Collagenase II	Gibco	17101015
DMEM (+ 4.5 g/L D-glucose, + L-Glut, − Sodium Pyruvate)	Gibco	11965-092
F-12 (+L-Glut)	Gibco	11765-054
FBS	Atlanta Biologicals	S11550
Fungizone	Gibco	15290-018
Human FGF-basic (FGF-2/bFGF) Recombinant Protein	ThermoFisher	13256-029
Insulin-transferrin-selenium-X (100x)	Gibco	41400045
MEM Non-essential Amino Acids	Gibco	11140
PBS, pH 7.4	Gibco	10010023
Penicillin-Streptomycin	Gibco	15140
StemPro™ Accutase™	ThermoFisher	A1110501
Triton X-100 (1%)	Gibco	HFH10
Quantification and Software
GraphPad	GraphPad Software, San Diego, California USA	www.graphpad.com
Image J	Schindelin et al.5	https://imagej.net/