Figure 3.

Early response of hematopoietic deletion of Tet2^−/− to ischemic kidney injury.

Mice were subjected to 32 minutes of ischemic injury to the left kidney with simultaneous removal of the right kidney. A) BUN levels and serum creatinine were significantly higher in Tet2^−/− mice. B) In kidneys of Tet2^−/− mice 8 days after injury, there was increased mRNA and immunoreactive protein kidney levels of the tubule injury markers Kim-1 and Ngal and inflammatory markers, NLRP3 and IL-1β. C) The tubule injury score was significantly higher in Tet2^−/− mice. D) Tet2^−/− kidneys had increased mRNA expression of proinflammatory cytokines, E) increased macrophage infiltration, indicated by increased immunoreactive expression of the macrophage marker F4/80, and F) isolated macrophages had increased mRNA expression of proinflammatory macrophages. G) There was increased kidney expression of Cd68 mRNA and increased CD68 colocalization with immunoreactive NLRP3 and IL-β. *p<0.05; **p<0.01; scale bar=50 μm.