Figure 1.

Conceptual diagram of experimental protocols. A: Timeline of behavioral testing. After a pre-training period before SAH induction or sham surgery, damage in the brain regions affected by SAH is assessed with a behavioral battery in which the indicated tests measure sensorimotor, memory and affective domains. (B) Subarachnoid hemorrhage is induced via endovascular perforation at the internal cerebral artery (ICA) bifurcation using a sharpened suture, after which hematoma forms in the basal subarachnoid space. Other abbreviations: ACA, anterior cerebral artery; BA, basal artery; MCA, medial cerebral artery (C) The top panel shows the early brain injury (EBI), which causes severe damage (red), and is associated with hematoma formation in the basal subarachnoid space damaging the piriform cortex, as well as increased intracranial pressure (ICP) damaging the motor, somatosensory, and piriform cortices, thalamus, and striatum. The lower panel shows the delayed cerebral ischemia (DCI), with more moderate damage (blue) developing in the hippocampus, white matter tracts, amygdala, and periventricular nuclei.