Figure 6. The β2 C31Y mutant shows increased susceptibility to proteasome-related inhibitors.

(a-d) 72-hour dose response assays were conducted on asynchronous C580Y, β2 C31Y, and β5 A20S parasites with (a) epoxomicin (EPX), (b) b-AP15, (c) chloroquine (CQ), and (d) methylene blue (MB). Data points on the graph indicate mean % inhibition ± S.E.M. (e-h) IC₅₀ values were calculated using non-linear regression analysis and plotted as bar graphs. Bar graphs depict mean IC₅₀ values ± S.E.M. Data shown is from four independent biological replicates. Statistical significance was determined using a Mann-Whitney U test. *p < 0.05; n.s. = not significant.