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[Preprint]. 2023 May 24:rs.3.rs-2921627. [Version 1] doi: 10.21203/rs.3.rs-2921627/v1

Figure 2. Validation using quantitative fluorescence in-situ hybridization confirms the existence of novel interneuron subclasses in the putamen.

Figure 2

A. Trackplot depicting raw UMI counts per nucleus for SST, NPY, DACH1, PTHLH, and PVALB in caudate and putamen. B. Proportion of cells positive for SST, SSTand NPY or SST, NPY, and DACH1 based on the total number of cells identified per donor (N = 6, putamen). C.Proportion of SST and NPY double-positive cells negative or positive for DACH1 (N = 6, putamen). D. Representative images for (left) single-, (middle) double- and (right) triple-positive cells from the same donor, scale bar 10 μm. E.Proportion of positive cells for PTHLH, PVALB, or PTHLH and PVALB based on the total number of cells identified per donor (N = 6, putamen). F. Proportion of PTHLH positive cells negative or positive for PVALB (N = 6, putamen). G.Representative images for (left and middle) single- or (right) double-positive cells from the same donor. White arrows indicate spot signal for PTHLH, asterisks mark autofluorescence due to lipofuscin, scale bar 10 μm.