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. 2023 Jun 7;9(23):eabq0712. doi: 10.1126/sciadv.abq0712

Fig. 4. Brain CD4+ T cells bear unique molecular signatures versus peripheral T cells after ICH.

Fig. 4.

CD3+ T cells were reclustered from CD45+ cell populations isolated from the spleen or brain tissues of C57BL/6 mice at 72 hours after ICH induced by autologous blood injection, based on the scRNA-seq data. (A) UMAP plot showing T cell subtypes of CD45+ cells from sham spleen, sham brain (CD45hi), ICH spleen, and ICH brain. (B) UMAP displaying expression of maker genes for T cell subtype identity. (C) Proportion of activated CD4+ T cells within total CD4+ T cells from sham spleen, sham brain (CD45hi), ICH spleen, and ICH brain. P = 0.000343 by Fisher’s exact test. (D) Heatmap showing relative average expression of differentially expressed genes in activated CD4+ T cells across ICH brain, ICH spleen, sham brain, and sham spleen group. (E) Selected significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways by gene set enrichment analysis between activated CD4+ T cells from ICH brain and those from sham brain. In (A) to (E), data were pooled from 15 mice in each group.