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. 2023 Jun 7;9(23):eabq0712. doi: 10.1126/sciadv.abq0712

Fig. 7. Depletion of CD4+ T cells ameliorates neurodeficits and ICH injury in mice.

Fig. 7.

C57BL/6 mice received intraperitoneal injection of 50 μg of anti-CD4 mAb or lgG control 24 hours before ICH induction with injection of autologous blood or collagenase. (A) Behavioral tests including modified neurological scale score (mNSS) and corner turning test were performed in indicated groups of autologous blood–induced ICH mice. (B) Multimodal 7-T magnetic resonance imaging (MRI) was used to visualize lesion (T2) and hematoma (SWI) in autologous blood–induced ICH mice. PHE volume was calculated by subtracting the hematoma volume from lesion volume. Representative MRI for hematoma and PHE in mice receiving anti-CD4 mAb or lgG following ICH induction for 24 hours. (C) Quantification of lesion volume, hematoma, and PHE volume in the indicated groups of autologous blood–induced ICH mice. (D) Behavioral tests in indicated groups of collagenase induced ICH mice. (E) Representative MRI for hematoma and PHE in mice receiving anti-CD4 mAb or lgG following ICH induction for 24 hours. Yellow shadows represent hematoma, and red lines delineate the lesion area. (F) Quantification of lesion volume and PHE volume in the indicated groups of collagenase-induced ICH mice. In (A) to (F), n = 7 mice per group. Two-way ANOVA followed by Tukey post hoc test. Means ± SD. *P < 0.05 and **P < 0.01.