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. 2023 Jun 7;9(23):eade8672. doi: 10.1126/sciadv.ade8672

Fig. 2. Surgically implanted abdominal imaging window allows longitudinal intravital imaging of pancreatic tumors in vivo.

Fig. 2.

(A and B) Computer-aided design of an abdominal PIW frame holding a circular glass coverslip (12 mm diameter). (C) Diagram of a mouse (using BioRender.com) indicating the anatomical location of the surgically implanted PIW, providing longitudinal imaging access to the pancreas in vivo. (D) Photo of the laser scanning confocal microscope with (E) a custom-designed, three-dimensionally (3D) printed stage insert to stabilize the animal for anesthesia and imaging. The stage is equipped with (1) a gas anesthesia port, (2) an electrical heating element, and (3) a holder to minimize motion artifacts during imaging and produce consistent images.