Table 1. Antibodies and dilutions used in the study.
N/A, not applicable.
| Primary antibody | Company, catalog no. | Dilution | Secondary antibody/reagent | Company, catalog no. | Dilution | |
|---|---|---|---|---|---|---|
| Western blot | Anti-GFP | Novus Biologicals, NB600-308 | 1:2000 | IRDye 680RD Detection Reagent | LI-COR Biosciences, 926-69100 | 1 μl/ml |
| Anti–β-tubulin | MilliporeSigma, 05-661 | 1:10,000 | ||||
| IVFM | APC-conjugated anti-CD31 | BD Biosciences, 551262 | 0.03 mg/ml | N/A | ||
| Tissue immunofluorescence | Anti-CD31 | BD Biosciences, 550274 | 1:100 | Anti-rat Alexa Fluor 488 | Thermo Fisher Scientific, A-11066 | 1:200 |
| Anti-PIMO | Hypoxyprobe, Pab2627 | 1:100 | Anti-rabbit Alexa Fluor 555 | Thermo Fisher Scientific, A-21428 | 1:200 | |
| Anti-CA9 | Novus Biologicals, NB100-479SS | 1:50 | Anti-rabbit Alexa Fluor 555 | Thermo Fisher Scientific, A-21428 | 1:200 | |
| Anti-GFP | Novus Biologicals, NB600-308SS | 1:500 | Anti-rabbit Alexa Fluor 555 | Thermo Fisher Scientific, A-21428 | 1:200 | |