Extended Data Fig. 8. Rescue experiment with either ARL6IP1 or the ARL6IP1-7KR variant.
a) In Arl6ip1 KO MEFs ER-phagy can be rescued by overexpression of either ARL6IP1 or ARL6IP1-7KR-HA. KO MEFs were transfected with the mCherry-GFP-FAM134B reporter and either ARL6IP1-HA or ARL6IP1-7KR-HA, fixed and stained for LC3B. Quantifications of LC3B/mCherry/GFP-positive puncta (autophagosomes) and LC3B/mCherry-positive but GFP-negative puncta (autolysosomes) per cell area (1 exp. with 15 cells analysed per genotype; Kruskal-Wallis test with Dunn`s multiple comparison test: autophagosomes WT versus KO p = 0.0002, KO versus ARL6IP1 rescue p = 0.0001, KO versus ARL6IP1-7KR rescue p = 0.0138; autolysosomes WT versus KO p = 0.001, KO versus ARL6IP1 rescue p = 0.0001, KO versus ARL6IP1-7KR rescue p = 0.0053). Scale bars: 20 µm. Quantitative data are shown as mean ± SEM.