Naïve +/+ and +/w mice were administered either PBS or 10 mg/kg of AMD3100 via the i.p. route. 2.5 h after injection, blood samples were collected and analyzed. (A) Total leukocyte count. (B) Absolute CD4+ and CD8+ T cell counts. (C) CD4/CD8 ratios. The Cxcr4 genotype (+, wild type; w, WHIM allele) code for all panels is shown as insets of the graphs. Data are summarized as the mean ± SEM of at least 10 mice per genotype from 10 independent experiments. ns: not significant, ****, p< 0.0001 as determined by the multiple t-test.