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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Structure. 2023 Apr 28;31(6):713–723.e3. doi: 10.1016/j.str.2023.04.005

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains
BL21(DE3) Agilent Technologies (formerly Stratagene) product number: 200131
Chemicals, peptides, and recombinant proteins
15N-labelled ammonium sulfate (15NH4)2SO4 Cambridge Isotope Laboratory NLM-713-1
D2O Cambridge Isotope Laboratory DLM-4-99-1000
SAM domain deletion mutant protein This paper N/A
15N-labeled SAM domain deletion mutant protein This paper N/A
Critical commercial assays
QuickChangeLightning Site directed Mutagenesis kit Agilent #210519
Oligonucleotides
Primers to generate deletion mutants, see Table S2 This paper N/A
Recombinant DNA
pET11a_1b0x_BDBV Schoeder et al.34 N/A
Plasmid DNA of deletion mutants This paper N/A
Software and algorithms
Rosetta software suite 3.13 version r280 8ee4f02ac5768a8a339ffada74cb0ff5f778b3e6 RosettaCommons www.rosettacommons.org
AlphaFold2 Jumper et al.51 https://github.com/deepmind/alphafold
TopSpin 3.6.2 Bruker https://www.bruker.com/de/products-and-solutions/mr/nmr-software/topspin.html
NMRViewJ SBGrid https://sbgrid.org/
PyMOL SBgrid https://sbgrid.org/
GraphPad Prism 9.1 GraphPad Software Inc. https://www.graphpad.com/scientific-software/prism/
MoltenProt Kotov et al.52 https://spc.emblhamburg.de/app/moltenprot
PSI-BLAST National Center for Biotechnology Information (NCBI) https://blast.ncbi.nlm.nih.gov/Blast.cgi
Python 3 Anaconda https://www.anaconda.com/
Jupyter notebook Anaconda https://www.anaconda.com/
JUFO Koehler-Leman et al.53 www.meilerlab.org
Computational Protocol Captures This paper N/A
Other
Prometheus Standard Capillaries Nanotemper PR-C002