Fig. 5.

Depletion of SNAP29 decreases the secretion of exosomal proteins, but not of annexins and autophagy-related proteins. sEVs were isolated by sequential centrifugation from SNAP29-depleted PC-3 cells and lysed. Releasing cells were also lysed and equal volumes of sEV or cell lysate loaded on SDS-PAGE gels. A Representative immunoblots and quantification of sEV proteins. B Representative immunoblotting and quantification of cellular proteins. Data shows mean ± SEM, n = 3–5. *P < 0.05 versus non-targeting control (non)