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. 2023 Jun 7;13:9226. doi: 10.1038/s41598-023-36202-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Dose–response and time courses following subcutaneous administration of fluorescein to female C57Bl/6J mice. (a) Representative scans and (b) quantification of fluorescein in the retina, vitreous and anterior chamber averaged after 30 min, from mice injected with 0.25, 0.50 and 1.0 mg of fluorescein subcutaneously. Time courses showing fluorescence levels in (c) the retina and vitreous, and (d) the anterior chamber of C57Bl/6J mice, 5–60 min after subcutaneous injection of 1 mg of fluorescein. N = 5–6 mice for dose-responses in (a and b), and N = 3 mice for time courses (c and d). R = retina; V = vitreous humour; AC = anterior chamber; SC = subcutaneous.