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. 2023 Jun 7;13:9226. doi: 10.1038/s41598-023-36202-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescein standard curves. Standard curves for fluorescein in (A) PBS and (B) plasma. Fluorescein standards were prepared in PBS or mouse plasma, then diluted further in PBS, before fluorescein concentration (ng/ml) was measured in a microcuvette. Regression analysis was carried out, with statistics shown on the graphs in the Figure.