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. 2023 May 17;159(6):465–475. doi: 10.1007/s00418-023-02195-4

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Fig. 6 — Demonstration of sulfatase activity in mouse liver with the azo dye method. Enzyme activity is detected in the hepatocyte cytoplasm and strongest in central region of the hepatic lobuli. Naphthol-AS-sulfate was used as enzyme substrate at pH 6.2 and Echtrot TR as diazo salt. Figure 3 from Gössner 1958