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The imbalance of macrophage polarization in T2DM fracture healing. (A) Localization of M1 (F4/80+ and iNOS+) macrophages. (B) M1 (F4/80+/iNOS+) macrophage quantization. n = 5. (C) Localization of M2 (F4/80+ and CD206+) macrophages. (D) M2 (F4/80+ and CD206+) macrophage quantization. n = 5. (E) iNOS, CD86, IL-1β, and TNF-α mRNA level. n = 3. (F) CD206, Arg-1, IL-10, and CD163 mRNA level. n = 3. Scale bar in all images: 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001.
