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RNA sequencing revealed M2-exosomes promote macrophages polarization through PI3K/AKT signaling pathway. (A) GO biological process analysis of upregulated genes in RM2. (B) KEGG analysis of upregulated genes in RM2. (C) The heatmap presents the gene levels associated with the PI3K/AKT pathways of M1 and RM2. (D) GSEA showed a significant increase of PI3K/AKT pathway gene enrichment score in RM2. (E) Western blot experiments showing the protein levels of PI3K, p-PI3K, AKT, and p-AKT.
