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. 2023 May 25;14:1192333. doi: 10.3389/fimmu.2023.1192333

Figure 4.

Figure 4

Time-course analysis of apoptosis mediated by CD146.CAR CIK cells. (A-C) TIME (A), MSCs (B) and HS-27A (C) were tested in a time course short-term cytotoxic assay (E:T ratio of 5:1) using Operetta CLS. A custom-made MatLab based software provide the image analyses to extract the populated area of the different dyes. Images show co-culture of different targets (TIME, MSCs and HS-27A) with CD146.CAR CIK cells. First row represents frame took at time 0, while second row reports frame took at the 8-hours end point.