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. 2023 May 25;17:1181804. doi: 10.3389/fnins.2023.1181804

Figure 2.

Figure 2

Tau mutations within or near the fourth MT binding repeat of tau have differential effects on tau-MT interactions. HEK293T cells were transfected to express WT tau in the 0N3R isoform and were (A) untreated or (B) treated with Paclitaxel in a cell-based MT binding assay. In the presence of Paclitaxel, the same assay was used on other tau mutations in the 0N3R isoform including (C) G335V, (D) S352L, (E) S356T, (F) V363A, (G) V363I, and (H) G366R. S = soluble and P = pellet. The immunoblots were probed with antibodies against β-tubulin (clone TUB 2.1) and for total tau (3026 antibody). The relative mobilities of molecular weight markers are shown on the left. (I) One-way ANOVA with Dunnett’s Test was performed with N = 6 for WT tau and N = 3 for tau mutations. **** = p < 0.0001, *** = p < 0.001, ** = p < 0.01, and ns = not statistically significant.