Figure 4.

S356T tau is more prone to form Thioflavin S-positive cellular aggregates compared to WT tau from cellular treatment with K19 seeds (A) HEK293T cells were transfected to express WT tau or S356T 0N3R tau and untreated to treated with K19 tau fibrils. The samples were stained for immunofluorescence with DAPI for nuclei (blue), tau antibody 3026 (red), and Thioflavin S for protein aggregates (green). Scale bar = 50 μm. (B) Graph shows comparison of significant Thioflavin S positive aggregates between different treatment groups. One-way ANOVA with Dunnett’s Test was performed with N = 6 for WT tau and S356T. **** = p < 0.001, ** = p < 0.01, ns = not statistically significant.