Table 1.
Gene panel selected for involvement in the Drosophila nitric oxide signaling pathway.
| Gene symbol | Full gene name | NO signaling pathway involvement | Function (localization) | Exemplary references |
|---|---|---|---|---|
| Nos | Nitric oxide synthase | Direct (signal production) | Production of nitric oxide signal | Regulski and Tully, 1995; Stasiv et al., 2001 |
| Gycalpha99B | Guanylyl cyclase α-subunit at 99B | Direct (signal reception) | NO receptor, soluble guanyl cyclase | Liu et al., 1995; Shah and Hyde, 1995; Morton et al., 2005 |
| Gycbeta100B | Guanylyl cyclase β-subunit at 100B | Direct (signal reception) | NO receptor, soluble guanyl cyclase | Shah and Hyde, 1995; Morton et al., 2005 |
| Gyc88E | Guanylyl cyclase at 88E | None | Constituent of atypical soluble guanyl cyclase heterodimers; NO-insensitive oxygen sensor | Langlais et al., 2004; Morton, 2004; Morton et al., 2005; Huang et al., 2007; Luo et al., 2009 |
| Gyc89Da | Guanylyl cyclase at 89Da | None | Constituent of atypical soluble guanyl cyclase heterodimers; NO-insensitive oxygen sensor | Morton, 2004; Morton et al., 2005; Vermehren-Schmaedick et al., 2010 |
| Gyc89Db | Guanylyl cyclase at 89Db | None | Constituent of atypical soluble guanyl cyclase heterodimers; NO-insensitive oxygen sensor | Langlais et al., 2004; Morton, 2004; Morton et al., 2005; Vermehren-Schmaedick et al., 2010 |
| Gyc32E | Guanylyl cyclase at 32E | None | Inferred membrane-associated guanyl cyclase receptor | Gigliotti et al., 1993 |
| Gyc76C | Guanylyl cyclase at 76C | None | Membrane-associated guanyl cyclase receptor | Liu et al., 1995; Overend et al., 2012; Chak and Kolodkin, 2014; Schleede and Blair, 2015 |
| CG42637 | – | None | Inferred membrane-associated guanyl cyclase receptor | FlyBase |
| CG34357 | – | None | Inferred membrane-associated guanyl cyclase receptor | FlyBase |
| CG33958 | – | None | Inferred membrane-associated guanyl cyclase receptor | FlyBase |
| CG31183 | – | None | Inferred membrane-associated guanyl cyclase receptor | FlyBase |
| CG10738 | – | None | Inferred membrane-associated guanyl cyclase receptor | FlyBase |
| CG3216 | – | None | Inferred membrane-associated guanyl cyclase receptor | FlyBase |
| For | Foraging | cGMP target | cGMP-dependent protein kinase | Osborne et al., 1997; MacPherson et al., 2004; Allen and Sokolowski, 2021; Kanoh et al., 2021 |
| Pkg21D | Protein kinase, cGMP-dependent at 21D | cGMP target | cGMP-dependent protein kinase | Foster et al., 1996; Vermehren-Schmaedick et al., 2010 |
| CG4839 | – | cGMP target | cGMP-dependent protein kinase | Wu et al., 2020 |
| CngA | Cyclic nucleotide-gated ion channel subunit A | cGMP target | cGMP-gated ion channel (plasma membrane) | Baumann et al., 1994 |
| CngB | Cyclic nucleotide-gated ion channel subunit B | cGMP target | cGMP-gated ion channel (plasma membrane) | Finn et al., 1998 |
| Cngl | Cyclic nucleotide-gated ion channel-like | cGMP target | cGMP-gated ion channel (plasma membrane) | Miyazu et al., 2000 |
| CG42260 | – | cGMP target | cGMP-gated ion channel (plasma membrane) | Vermehren-Schmaedick et al., 2010; Lee et al., 2020 |
| Pde1c | Phosphodiesterase 1c | cGMP target | cGMP phosphodiesterase (cytosolic) | Day et al., 2005 |
| Pde6 | Phosphodiesterase 6 | cGMP target | cGMP phosphodiesterase (cytosolic) | Day et al., 2005 |
| Pde9 | Phosphodiesterase 9 | cGMP target | cGMP phosphodiesterase (cytosolic) | Day et al., 2005 |
| Pde11 | Phosphodiesterase 11 | cGMP target | cGMP phosphodiesterase (cytosolic) | Day et al., 2005 |
| Orco | Olfactory receptor co-receptor | ? | Olfactory co-receptor (OR subsystem only) | Larsson et al., 2004; Benton et al., 2006 |
| Or67d | Odorant receptor 67d | ? | Pheromone receptor (at1 sensilla) | Kurtovic et al., 2007; Wang and Anderson, 2010 |
| Ir8a | Ionotropic receptor 8a | ? | Olfactory co-receptor (subset of IR subsystem only) | Benton et al., 2009; Abuin et al., 2011 |
| Act5C | Actin 5C | None | Housekeeping, cytoskeleton component (pancellular) | Lü et al., 2018 |
| Cam | Calmodulin | Constitutive NOS activation | Housekeeping, intermediate messenger (pancellular) | Regulski and Tully, 1995; Mukunda et al., 2016; Jain et al., 2021 |
| Gapdh1 | Glyceraldehyde 3 phosphate dehydrogenase 1 | None | Housekeeping, glycolysis (pancellular) | Lü et al., 2018 |