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. 2023 Jun 7;6(8):e202302025. doi: 10.26508/lsa.202302025

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© 2023 Ohori et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S5. — Whole-mount staining of stage-16 embryos with the widely used tracheal marker 2A12 antibody. (A) Oregon R (non-treated) embryos. (B) 69B-GAL4>UAS-Bnl WT embryos. (C) 69B-GAL4>UAS-Bnl p.Glu324Lys embryos. (A, B, C) The numbers showing a dorsal trunk (DT) defect were checked and are described at the bottom. 69B-GAL4>UAS-Bnl WT embryos (B) lost the normal pattern of branching, with fine branches growing out in random directions (white arrows). These tracheal abnormalities were not observed in Oregon R (A) and 69B-GAL4>UAS-Bnl Glu324Lys embryos (C). Scale bar: 50 μm.