Figure 5.

SCFAs upregulate the antigen presenting capacity of primary mouse CRC organoids. Primary CRC organoids were derived from tumors induced by repeated doses of azoxymethane (10 weekly doses of 10 mg/kg azoxymethane) to wild type C57BL/6 mice. Mlh1 was then knocked down by stably transducing with shRNA (Mlh1^-/- ) to create an MSI variant. CIN variants were made using a scrambled sequence (Ctl). Organoids were then treated with 50 mM butyrate, 50 mM propionate or a combination of the two for 24 h. Organoid cells were harvested immediately after the stimulation (“Initial”) or were cultured a further 24 h in the absence of any treatment (“Sustained”). Expression of ISGs (A) and antigen processing and presentation machinery (B) were the analyzed by qPCR. For all panels, n = 3 experimental repeats with 3 biological replicates per experiment. Representative graphs from a single experiment are shown. For all panels, relative to the untreated control: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.