Figure 6.

SCFAs upregulate the antigen presenting capacity of primary CRC patient organoids. Primary CRC patient organoids were derived from two separate patients, CRC-A (A) and CRC-B (B). For organoids from each patient, MLH1 was then knocked down by stably transducing with shRNA (MLH1^-/- ) to create an MSI variant. CIN variants were made using a scrambled sequence (Ctl). Organoids were then treated with 25 mM butyrate, 25 mM propionate or a combination of the two for 24 h. Organoid cells were harvested immediately after the stimulation and gene expression was analyzed by qPCR. For all panels, n = 3 experimental repeats with 2 biological replicates per experiment. Representative graphs from a single experiment are shown. For all panels, relative to the untreated control: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.