Fig. 5.

Comparison of root and shoot expression pattern of nitrogen metabolism genes in wild-type (WT) and osnrt2.4 knockdown lines (r1 and r2). Rice seedlings were grown in a hydroponic system. Thirteen-day-old seedlings were transferred into nutrient solution without nitrogen for 4 days (starvation) or with 0.1mM N-NO₃⁻ for 4 days (low nitrogen). Total RNA was isolated from roots at 04.00h (04) (light start). OsGS1;1, OsGS2, and OsNIA1 relative expression in roots (a, b, and c) and shoots (d, e, and f). The expression levels were normalized to the expression of the OsUBC and OseEF-1α reference genes. The experiment was performed using four biological replicates for each genotype. Bars indicate the standard error of the mean (n =16). Asterisk (*) indicates statistically significant differences between WT and two mutant lines by the Dunnett's test (p<0.05)