Table 5.

Genotyping cost (US$ per sample) for different sequencing technologies

Procedure	GBTS a			GBS b	DNA chips c
	40K	20K	10K
DNA extraction	0.44	0.44	0.44	0.44	0.44
Library construction	2.94	2.94	2.94	5.88	29.41
Probe hybridization	2.21	2.21	2.21	0.00
Sequencing	4.41	2.94	1.47	4.41
Bioinformatics analysis	0.74	0.29	0.15	0.74
Labor	1.47	1.47	1.47	1.47	1.47
Depreciation cost	1.47	1.03	0.59	1.47	1.47
Total	13.68	11.32	9.26	14.41	32.79

^aGBTS, genotyping by target sequencing

^bGBS, genotyping by sequencing, where the sequence depth is 2×

^cDNA chips, chips containing 50K SNP markers with a genotyping cost of $32.79