Table 2.
Genotypic analysis of BnaAP39s mutants in T0 and T1 generations. (The red labeled ID indicated the two individual lines in this study)
| Plant ID | Generation | genotype at targets of BnaA06.AP39 | genotype at targets of BnaC03.AP39 | ||
|---|---|---|---|---|---|
| sgRNA5 | sgRNA6 | sgRNA5 | sgRNA6 | ||
| cr-bnaap39-L1 | T0 | WT | Hetero | WT | Hetero |
| cr-bnaap39-L2 | T0 | WT | WT | WT | Hetero |
| cr-bnaap39-L3 | T0 | Hetero | WT | Hetero | Hetero |
| cr-bnaap39-L4 | T0 | Hetero | Hetero | Hetero | Hetero |
| cr-bnaap39-L4-1 | T1 | Hetero | Homo (i) | Hetero | Homo (i) |
| cr-bnaap39-L4-2 | T1 | Hetero | Homo (i) | Hetero | Homo (i) |
| cr-bnaap39-L4-3 | T1 | Hetero | Homo (i) | Hetero | Homo (i) |
| cr-bnaap39-L4-4 | T1 | Hetero | Homo (i) | Hetero | Homo (i) |
| cr-bnaap39-L4-5 | T1 | Hetero | Homo (i) | Hetero | Homo (i) |
| cr-bnaap39-L5 | T0 | WT | WT | Hetero | Hetero |
| cr-bnaap39-L6 | T0 | Hetero | Hetero | Hetero | Hetero |
| cr-bnaap39-L7 | T0 | Hetero | Hetero | Hetero | Hetero |
| cr-bnaap39-L7-1 | T1 | Hetero | Homo (i) | Hetero | Hetero |
| cr-bnaap39-L7-2 | T1 | WT | WT | WT | Hetero |
| cr-bnaap39-L7-3 | T1 | Hetero | Hetero | Hetero | Hetero |
| cr-bnaap39-L7-4 | T1 | Hetero | Hetero | Hetero | Hetero |
| cr-bnaap39-L7-5 | T1 | Hetero | WT | Hetero | Hetero |
| cr-bnaap39-L8 | T0 | Hetero | Hetero | Hetero | Hetero |
| cr-bnaap39-L8-1 | T1 | WT | WT | WT | Hetero |
| cr-bnaap39-L8-2 | T1 | WT | WT | WT | WT |
| cr-bnaap39-L8-3 | T1 | Hetero | Hetero | Hetero | Hetero |
| cr-bnaap39-L8-4 | T1 | Hetero | WT | Hetero | Hetero |
| cr-bnaap39-L8-5 | T1 | WT | WT | WT | Hetero |
| cr-bnaap39-L9 | T0 | Hetero | WT | Hetero | Hetero |
| cr-bnaap39-L9-1 | T1 | Homo (id) | WT | Homo (id) | Homo (i) |
| cr-bnaap39-L9-2 | T1 | Homo (id) | WT | Homo (id) | Homo (i) |
| cr-bnaap39-L9-3 | T1 | Homo (id) | WT | Homo (id) | Homo (i) |
| cr-bnaap39-L9-4 | T1 | Homo (id) | WT | Homo (id) | Homo (i) |
| cr-bnaap39-L9-5 | T1 | Homo (id) | WT | Homo (id) | Homo (i) |
Hetero, heterozygous; Homo, homozygous; wt, no mutations were identified; #d, #of bp deleted from a target site; #i, # of bp inserted at target site; #s, #of bp substitute at target site