Glucose bolus stimulates FABP4 secretion in vivo. A: Glucose excursion curve during OGTT (N = 9–10/group; two-way ANOVA). B: Corresponding plasma insulin levels during OGTT (N = 9–10/group; two-way ANOVA). C: Plasma FABP4 in ATGLfl/fl and ATGLAdpKO mice under ad libitum fed, 10 min following ISO injection (10 mg/kg), 16 h fasting, and 10 min following glucose injection (1 g/kg) (N = 10–21/group; Student’s t-test). D: Plasma glycerol and (E) plasma FABP4 levels in ATGLAdpKO and HSLAdpKO and respective floxed control mice injected with ISO (10 mg/kg) (N = 9–16/group; two-way ANOVA). F: Plasma glycerol and (G) plasma FABP4 levels in MGLfl/fl and MGLAdpKO mice injected with ISO (10 mg/kg) (N = 5/group; two-way ANOVA). H: Schematic illustration of the protocol for serum induction of lipolysis in 3T3-L1 cells. I: Plasma FABP4 levels in mice 10 min following injection of PBS or ISO (10 mg/kg) (N = 4–7/group; one-way ANOVA). J: Quantification of FABP4 in conditioned media from 3T3-L1 adipocytes treated with control, ISO (1 μM), or 10% serum from mice in (I) (N = 6–14/group; one-way ANOVA). All mouse experiments performed in 8- to 10-week-old male mice. Data are presented as mean ± SEM.