FIGURE 6.

Transforming growth factor (TGF)β1 drives endothelial cell (EC)-like myofibroblasts towards myofibroblast differentiation. a) Fluorescence-activated cell sorting of whole lung cells of Sm22a^creRosa^tdTomato mice. b) Gene expression of tdTomato-negative and VE-cadherin-positive (tdTomato⁻VE-cadherin⁺), tdTomato⁺VE-cadherin⁺ and tdTomato⁺VE-cadherin⁻ cells isolated from lungs of Sm22a^creRosa^tdTomato mice (n=6). c) Gene expression of tdTomato⁻VE-cadherin⁺, tdTomato⁺VE-cadherin⁺ and tdTomato⁺VE-cadherin⁻ cells after matrix Gla protein (MGP) deletion with siRNA and treated with or without TGFβ1 (1 ng·mL⁻¹) (n=6). Data were analysed for statistical significance by ANOVA with post hoc Tukey's test. The bounds of the boxes are upper and lower quartiles with data points; the line in the box is median; error bars are maximal and minimal values. Fn1: fibronectin 1; SMMHC: smooth muscle myosin heavy chain; vWF: von Willebrand factor. ***: p<0.0001.