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. 2023 Jun 8;61(6):2201799. doi: 10.1183/13993003.01799-2022

FIGURE 8.

FIGURE 8

Berbamine ameliorates pulmonary fibrosis in mouse models. a) Uniform manifold approximation and projection (UMAP) and differential gene expression between endothelial cell (EC)-like myofibroblasts and ECs. b) Gene expression in tdTomato+VE-cadherin+ cells isolated from the lungs of SM22acreRosatdTomato mice and treated with berbamine (20 µM) with transfection of matrix Gla protein (MGP) siRNA or scrambled siRNA (SCR) (n=6). c) Masson's trichrome (MT) staining of pulmonary tissues from VE-cadherincreMgpflox/flox and Sm22acreMgpflox/flox mice after berbamine treatment (100 ng·g−1, daily) (n=12). Scale bars=100 µm. d) Quantification of MT staining and expression of Col3a1 and fibronectin 1 (Fn1) in pulmonary tissues from VE-cadherincreMgpflox/flox and Sm22acreMgpflox/flox mice after berbamine treatment (n=9 for quantification of MT and n=6 for gene expression). b) and d) were analysed for statistical significance by ANOVA with post hoc Tukey's test. The bounds of the boxes are upper and lower quartiles with data points; the line in the box is median; error bars are maximal and minimal values. **: p<0.001, ***: p<0.0001.