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. Author manuscript; available in PMC: 2023 Jun 8.
Published in final edited form as: Science. 2022 Jul 22;377(6604):eabm5551. doi: 10.1126/science.abm5551

Figure 3. Native expression of the lysosomal transporter SLC46A3 predicts NP-cell interaction for liposome formulations.

Figure 3.

(A) Univariate analysis identified SLC46A3 expression as strongly inversely correlated with liposome association, regardless of liposomal surface modification. (B) Using linear regression to evaluate the biomarker relationship across core formulations revealed SLC46A3 expression was inversely correlated with NP association in liposome-cell line pairs (p < 0.001) but not PLGA- and PS-cell line pairs (p > 0.05); n=488 for each plot. (C) Cell lines in the nanoPRISM pool exhibited a range of native SLC46A3 expression and a log linear correlation with uptake of bare liposomes. (D) This same correlation was also exhibited when assessing liposome-cell associations via flow cytometry in a non-pooled fashion (p = 0.025). Cell lines in red were not part of the pooled PRISM screen. Data represented in D are shown as the mean and standard deviation of four biological replicates. Error bars are not shown when smaller than data points.