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. 2023 Jun 7;14:159. doi: 10.1186/s13287-023-03387-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — eMSCs suppressed the tumor-related stemness of EC cells. A1-A3 eMSCs inhibited sphere-forming ability of EC organoids and cell lines. Single cell suspensions of EC cells were seeded in Matrigel and treated with NM or CM from eMSCs for 15 days. The numbers of visible spheres were calculated. Original magnification, 4 × ; Scale bar, 400 μm. B1-B3 eMSCs inhibited the transcriptional levels of EC stemness-associated genes (ALDH1, BMI1, and NANOG) both in EC organoids and cell lines. CM derived from eMSCs was used to treat EC organoids for 96 h or EC cell lines for 48 h. EC cells were then harvested for qRT-PCR analysis. NM, normal medium; CM, conditioned medium; Data were analyzed by unpaired t-test (A-B). *P < 0.05; **P < 0.01; ***P < 0.001