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. 2023 Jun 7;14:156. doi: 10.1186/s13287-023-03386-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Impact of culture conditions on gene expression and engraftment. A Volcano plot shows the expression profile of 82 genes expressed after culture with XF compared to FBS media using the Human Mesenchymal Stem Cell RT² Profiler PCR Array (PAHS-082, SABiosciences, Qiagen, Hilden, Germany). The genes with fold changes higher than the cut-off value (fold change > 2 with p < 0.05) were selected. B Fourteen transcripts (INFG, SOX2, WNT3A, FGF10, JAG1, BDNF, ICAM1, MCAM, KITLG, ITGA6, GDF15, ENG, SLC17A5 and FGF-2) were upregulated during culture in XF media and four transcripts (NES, GDF7, ANPEP and RUNX2) were downregulated. C Relative mRNA expression of the gene LOXL2, a gene our previous data showed upregulation in MDS. D Comparison of the mean hCD45 + cell engraftment from MDS patient material co-transplanted with MSCs expanded with FBS or XF media. There was no difference in human engraftment even for the samples from the same patient and time point (E)