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. 2023 Jun 8;56:30. doi: 10.1186/s40659-023-00436-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Mitophagy is unchanged by DCA and CA in C₂C₁₂ myotubes. a–c C₂C₁₂ myoblasts were differentiated for 5 days until forming myotubes. Then, myotubes were incubated with 120 μM of DCA or 500 μM of CA for 48 h. a After treatment with BA, cell culture was co-incubated with Mitotracker green FM (first panel) and Lysotracker Red DND-99 (second panel) probes. The co-localization (third panel and forth panel) between Mitotracker green and Lysotracker signals was determined as a parameter of mitophagy. Scale bar: 10 μm. Co-localization degree of fluorescent signals was calculated by Manders' coefficient using the JACoP plugin by ImageJ for (b) DCA and (c) CA. The values indicate the mean ± SEM of independent random fields. Scale bar:10 μm. (n = 3 independent experiments, *p < 0.05. t-test)