Table 2. Summary of Apparent Kinetic Parameters Determined for the Wild-Type and Variant Enzymes Studied in This Work.

enzyme and substrate	KMapp (μM)	kcatapp (min–1)	kcatapp/KMapp (M–1 s–1)
TsaM, VanB, and vanillate	182 ± 3.7	1.8 ± 0.7	230 ± 48
TsaM variant 14, VanB, and vanillate	13 ± 0.8	3.8 ± 0.2	5100 ± 530
TsaM variant 15, VanB, and vanillate	20 ± 1.2	3.7 ± 0.2	3200 ± 260
VanA, VanB, and vanillate	6.4 ± 0.6	3.4 ± 0.3	8900 ± 800
TsaM, VanB, and dicamba	N/A	N/A	N/A
TsaM variant 15, VanB, and dicamba	100 ± 9.2	1.8 ± 0.2	300 ± 43
TsaM variant 18, VanB, and dicamba	42 ± 6.2	2.8 ± 0.3	1100 ± 200
TsaM variant 18, DdmA, DdmB, and dicamba	39 ± 7.2	2.7 ± 0.2	1200 ± 240
DdmC, DdmA, DdmB, and dicamba	28 ± 3.4	2.8 ± 0.1	2100 ± 240
DdmC, VanB, and dicamba	22 ± 4.1	2.8 ± 0.3	2300 ± 410