. 2023 May 10;62(11):1689–1705. doi: 10.1021/acs.biochem.3c00129

Table 1. Apparent Dissociation Constants (K_d^app) and Hill Coefficients (n_H) for the OR-Metal Ion Complex, Based on Circular Dichroism (CD) and Fluorescence Quenching Experiments.

metal ion	method	buffer	K_d1^app [μM]	n_H1	K_d2^app [μM]	n_H2	K_d3^app [μM]	n_H3
Cu(II)	CD at 208 nm	10 mM NaH₂PO₄, pH 7.5	0.52 ± 0.31	0.97 ± 0.48	5.57 ± 0.11	2.97 ± 0.25
Cu(II)	CD at 224 nm	10 mM NaH₂PO₄, pH 7.5	0.24 ± 0.03	1.31 ± 0.20	7.84 ± 0.25	1.81 ± 0.14	18.0 ± 0.7	4.50 ± 0.41
Zn(II)	CD at 224 nm	10 mM NaH₂PO₄, pH 7.5	0.64 ± 0.17	1.28 ± 0.26	5.61 ± 0.37	5.11 ± 2.02
Cu(II)	fluorescence at 355 nm	10 mM NaH₂PO₄, pH 7.5	4.29 ± 0.06	1.5 ± 0.03
Cu(II)	fluorescence at 355 nm	10 mM MES, pH 7.5	4.5 ± 0.1	1.61 ± 0.05

Table 1. Apparent Dissociation Constants (Kdapp) and Hill Coefficients (nH) for the OR-Metal Ion Complex, Based on Circular Dichroism (CD) and Fluorescence Quenching Experiments.