Table 3. Affinities of the FERM Domains of the ERMs for a Representative Set of Ligands Determined through Competition FP Experiments Using FITC-KIRREL3 as the Probe Peptide (except for HIF1A)a.
| unlabeled peptides in competition with FITC-KIRREL3 | ezrin KD′ (μM) | radixin KD′ (μM) | moesin KD′ (μM) | |
|---|---|---|---|---|
| BOC1000–1015 | LYTLPDDSTHQLLQPH | 8.0 ± 0.1 | 17 ± 2 | 18 ± 1 |
| CAMK2B13–28 | EYQLYEDIGKGAFSVV | 26 ± 0.6 | 6.8 ± 0.4 | 96 ± 4 |
| GCM2274–289 | SYELANPGYTNSSPYP | 205 ± 11 | 85 ± 3 | 38 ± 3 |
| ICAM3516–531 | SGSYHVREESTYLPLT | - | - | 113 ± 3 |
| LATS1421–436 | LYNISVPGLQTNWPQS | - | - | 149 ± 5 |
| MISP595–610 | ITGSYSVSESPFFSPI | - | - | 2.3 ± 0.1 |
| PDCD6IP853–868 | SYPFPQPPQQSYYPQQ | - | - | 35.2 ± 0.2 |
| RIMS11405–1420 | MYTLEHNDGSQSDTAV | - | - | 564 ± 5 |
| RORA185–200 | TYNISANGLTELHDDL | - | - | 134 ± 3 |
| HIF1A37–52 | FYELAHQLPLPHNVSS | 3.0 ± 0.4b |
a
- Not measured.
b
Determined using FITC-ZNF622 as the probe peptide.