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. Author manuscript; available in PMC: 2024 May 4.
Published in final edited form as: Cell Stem Cell. 2023 May 4;30(5):632–647.e10. doi: 10.1016/j.stem.2023.04.006

Key resources table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
CASPR Generous gift from Dr. Joel Black N/A
CD49D Biolegend Cat#304301;
RRID:AB_314427
CHAT Proteintech Cat#20747–1-AP;
RRID:AB_10898169
CHAT Sigma Cat#AB144P;
RRID:AB_2079751
GFAP Abcam Cat#ab4674;
RRID:AB_304558
Kv1.2 Generous gift from Dr. Joel Black N/A
MAG Millipore Cat#LS-C279052-200
MBP Millipore Cat#MAB386;
RRID:AB_94975
MPZ Abcam Cat# ab39375;
RRID:AB_881430
NFH Encor Cat#RPCA-NF-H;
RRID:AB_2572360
NFkB p65 Invitrogen Cat#710048;
RRID:AB_2532539
PanNa Generous gift from Dr. Joel Black N/A
PMP22 Novus Biologicals Cat#NB110–59086;
RRID:AB_892372
S100B Thermo Scientific Cat#RB-9018-P0;
RRID:AB_149782
SC101 Takara Cat#Y40400;
RRID:AB_2895096
TUBB3 Millipore Sigma Cat#ab9354;
RRID:AB_570918
TUBB3 Biolegend Cat#801202;
RRID:AB_10063408
donkey a-goat AF488 Invitrogen Cat#A11055;
RRID:AB_2534102
donkey a-goat AF568 Invitrogen Cat#A11057;
RRID:AB_142581
donkey a-goat AF647 Invitrogen Cat#A21447;
RRID:AB_141844
donkey a-mouse AF488 Invitrogen Cat#A21202;
RRID:AB_141607
donkey a-mouse AF568 Invitrogen Cat#A10037;
RRID:AB_2534013
donkey a-mouse AF647 Invitrogen Cat#A31571;
RRID:AB_162542
donkey a-rabbit AF488 Invitrogen Cat#A11008;
RRID:AB_143165
donkey a-rabbit AF568 Invitrogen Cat#A10042;
RRID:AB_2534017
donkey a-rabbit AF647 Invitrogen Cat#A31573;
RRID:AB_2536183
goat a-chicken AF488 Invitrogen Cat#A11039;
RRID:AB_142924
Chemicals, peptides, and recombinant proteins
Accutase Innovative Cell Technologies Cat# AT104
Araldite 6005 EMS Cat#10920
Ascorbic acid Sigma-Aldrich Inc Cat#A4034
B27 Life Technologies Cat#17504044
Cytofix/Cytoperm buffer BD Bioscience Cat#554722
Perm/Wash buffer BD Bioscience Cat#554723
Benzyldimethylamine EMS Cat#11400
BMP4 R&D Systems Cat#314-BP
BSA Thermo Scientific Cat#23209
Bupropion, HCl Sigma-Aldrich Inc Cat#B102
cAMP Sigma-Aldrich Inc Cat#D0627
CHIR99021 Tocris Bioscience Cat#4423
DAPI Sigma-Aldrich Inc Cat#D9542–5MG
Dibutyl Phthalate Ted Pella Cat#18231
DMEM/F12 Thermo Fisher Scientific Cat#21331020
Dodecenyl Succinic Anhydride EMS Cat#13700
Essential 6 (E6) medium Thermo Fisher Scientific Cat#A1516401
Essential 8 (E8) Flex medium Thermo Fisher Scientific Cat#A2858501
0.5 M EDTA, pH 8.0 Thermo Fisher Scientific Cat#15575020
FGF2 R&D Systems Cat#233-FB-001MG/CF
Fibronectin Corning Cat#356008
Fluo-4 AM Life Technologies Cat#F14201
GDNF Peprotech Cat#450–10
Geltrex Thermo Fisher Scientific Cat# A1413302
glucose Sigma-Aldrich Inc Cat#5767
L-Glutamine Gibco Cat#25030–164
Glutagro Corning Cat#25–015-CI
Karnovsky's Fixative EM Grade EMS Cat#15732–10
KSR Life Technologies Cat#10828–028
Laminin Cultrex 3400–10
LDN193189 Stemgent 04–0074-base
Lead Citrate Eastman Cat#11534
Matrigel BD Biosciences Cat#354234
MEM non-essential amino acids (NEAA) Corning Cat#25–025-CI
Mouse embryonic fibroblast (MEF) Gibco Cat#A34959
Neurobasal medium Gibco Cat#21103049
NNeurobasal medium (no glucose, no pyruvate) Gibco Cat#A2477501
N2 CTS Cat#A1370701
NRG1 R&D Systems Cat#378-SM-025
Osmium tetroxide Electron Microscopy Sciences (EMS) Cat#19150
Paraformaldehyde, 4% solution SCBT Cat#:sc-281692
Pluronic®-F127 Life Technologies Cat#P3000MP
Poly/Bed 812 Polysciences Cat#08791–500
Poly-L-ornithine hydrobromide Sigma Cat#P3655
Prestwick Library of FDA approved drugs Prestwick Chemical Libraries N/A
Propylene oxide EMS Cat#20401
SB431542 R&D Systems Cat#:1614
Schwann Cell Medium Sciencell Cat#1701
STZ Sigma-Aldrich Inc Cat#85882
triton X-100 Sigma-Aldrich Inc Cat#T8787
0.05% Trypsin/0.53 mM EDTA Fisher Scientific Cat#MT25052CI
Uranyl Acetate Ted Pella Cat#19481
Critical commercial assays
Chromium Next GEM Single Cell 2c Kit v3.1 10X Genomics Cat#1000121
CytoTox 96 cytotoxicity assay kit Promega Cat#G1780
D-sorbitol Assay kit Abcam Cat#ab118968
eBioscience™ Permeabilization Buffer (10X) Invitrogen Cat#00–8333-56
Fructose assay kit Abcam Cat# ab83380
Human cell surface marker screening panel BD Cat#560747
QuantiTect SYBR Green PCR mix Qiagen Cat#204148
MitoSOX™ Mitochondrial Superoxide Indicators Invitrogen Cat# M36008
RNeasy RNA purification kit Qiagen Cat#74106
Superscript II Reverse Transcriptase Life Technologies Cat#18064–014
Deposited data
Bulk and single cell RNA sequencing data on hPSC-derived Schwann Cells This study GEO: GSE195730
Targeted metabolomics data This study NMDR: ST002540
Experimental models: Cell lines
Human embryonic stem cells, H9 WiCell RRID: CVCL_1240
H9-derived SOX10::GFP reporter line Chambers et al.11 N/A
H9 RFP line Fattahi et al.12 N/A
Human induced pluripotent stem cells, WTC11 Coriell Institute RRID: CVCL_Y803
Experimental models: Organisms/strains
8 weeks old male C57BL6 mice The Jackson Laboratory RRID:IMSR_JAX:00 0664
10 weeks old male Sprague Dawley rats Charles River RRID:RRRC_00239
Oligonucleotides
Oct6 Qiagen QuantiTect Primer Assays
Egr2 Qiagen QuantiTect Primer Assays
Gfap Qiagen QuantiTect Primer Assays
Pax3 Qiagen QuantiTect Primer Assays
S100b Qiagen QuantiTect Primer Assays
Plp1 Qiagen QuantiTect Primer Assays
Pmp22 Qiagen QuantiTect Primer Assays
Erbb3 Qiagen QuantiTect Primer Assays
Sox10 Qiagen QuantiTect Primer Assays
Mbp Qiagen QuantiTect Primer Assays
Software and algorithms
iPAGE gene ontology analysis Goodarzi et al.31 https://tavazoielab.c2b2.columbia.edu/iPAGE/
SEA computational tool Keiser et al. 32 https://bkslab.org/
STRING v11 protein-protein interaction (PPI) network analysis Szklarczyk et al.33 https://string-db.org/
Flow Jo FlowJo LLC https://www.flowjo.com/
Prism 9 GraphPad Software https://graphpad.com
Python (version 3.8.8) Python https://www.python.org/downloads/
Pandas (version 1.4.1) Python Package Index https://pypi.org/project/pandas/
NumPy (version 1.22.2) Python Package Index https://pypi.org/project/numpy/1.22.2/
Seaborn (version 0.11.2) Python Package Index https://pypi.org/project/seaborn/
Metamorph Software Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy
Tophat v.2.1.1 Johns Hopkins University Center for Computational Biology https://ccb.jhu.edu/software/tophat/index.shtml
R package: HTSeq v0.12.4 CRAN package repository https://cran.r-project.org/web/packages/
R package: DESeq2 v1.30.0 CRAN package repository https://cran.r-project.org/web/packages/
R package: bestNormalize (v1.4.0) CRAN package repository https://cran.r-project.org/web/packages/bestNormalize/index.html
R (v4.1.3) The R Project forStatisticalComputing https://www.r-project.org/
R (v4.2.0) The R Project forStatisticalComputing https://www.r-project.org/
R package: nVennR (version 0.2.3) Github Inc https://github.com/cran/nVennR
R package: Seurat v4.0 CRAN package repository https://cran.r-project.org/web/packages/
R package: MSigDB v1.6.0 CRAN package repository https://cran.r-project.org/web/packages/
R package: BiomaRt v2.54.0 CRAN package repository https://cran.r-project.org/web/packages/
R package: SingleCellNet v0.4.1 Tan and Cahan et al.26 https://github.com/
g:Profiler Raudvere et al.63 https://biit.cs.ut.ee/gprofiler/gost
Adobe Illustrator Adobe https://www.adobe.com/products/illustrator/free-trial-download.html
Other
Molecular Signatures Database MSigDB v6.0 https://software.broadinstitute.org/cancer/software/gsea/wiki/index.php/MSigDB_v6.0_Release_Notes
Previously published bulk RNA-seq dataset Tasdemir-Yilmaz et al.25 GEO: GSE172110
Previously published bulk RNA-seq dataset Eraslan et al.27 https://singlecell.broadinstitute.org/single_cell/study/SCP1479/single-nucleus-cross-tissue-molecular-reference-maps
Previously published bulk RNA-seq dataset Fattahi et al.12 GEO/GSE66148