Fig. 3: NK and senescence markers within T_N and T_EMRA.

a) UMAP plot of single-cell sorted IL-7R⁺CD8⁺ and IL-7R⁻CD8⁺ T cells from six healthy older donors. Highlighted clusters were considered as T_N (C0, C4 and C8) and T_EMRA (C1, C2 and C6) compartments. b) UMAP plot showing re-clustering of selected T_N (C0, C4, and C8) and T_EMRA (C1, C2, and C6) CD8⁺ cells from a) (n = 39,634 cells). c) UMAP plots representing the expression values of NK-related genes in re-clustered T_N and T_EMRA CD8⁺ cells from b). Scales show low expression (yellow) to high expression (red). d) Violin plots of NK-related gene expression determined by scRNA-seq in T_N and T_EMRA CD8⁺ T cells clustered as in b) (means, range, and distribution of individual data; n = 39,364 single cells). e) UMAP plots showing expression of senescence-associated determined by scRNA-seq in T_N and T_EMRA CD8⁺ T cells re-clustered as in b). Scales show low expression (yellow) to high expression (red). Other aliases or CD numbers of some gene products are shown in brackets. f) Violin plots of senescence scores calculated based on the average normalized expression of each senescence-associated gene across T_N and T_EMRA CD8⁺ cells clustered as in b) (means, range, and distribution of individual data; n = 39,364 single cells). The gene lists used to define NK- and senescence-scores are given in Supplementary table 4).