Extended data 1: CD8⁺ T cell gating and NKR expression.

a) Representative flow cytometry plots showing T cell gating and NKR expression on peripheral blood lymphocytes, specifically focusing on CD8⁺ T cell subsets stratified by the expression of CD27/CD45RA in healthy donors. Defined subsets are CD27⁺CD45RA⁺ T_N, CD27⁺CD45RA⁻ T_CM, CD27⁻CD45RA⁻ T_EM, and CD27⁻CD45RA⁺ T_EMRA cells. b) Confirmation of expression of CD57, KLRG1, CD244, NKG2D, NKG2C, and KIR2DL on T_N (naïve), T_CM, T_EM, and T_EMRA CD8⁺ T cell subsets. Numbers in quadrants represent percentages of cells in each subset. Numbers above the histograms indicate the MFI. c) Flow cytometry gating of CD8⁺ T cells to confirm CD27 and CD28 expression in subpopulations based on CD27/CD45RA gating.