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. Author manuscript; available in PMC: 2023 Jun 8.
Published in final edited form as: Nature. 2022 Dec 7;612(7940):503–511. doi: 10.1038/s41586-022-05495-2

Fig. 3. PCDHγ removal causes a lateral clustering of clonally related excitatory neurons.

Fig. 3.

a, Diagram of the experimental procedure of MADM-based clonal analysis in the WT and Pcdhg-cKO neocortices. b,c, Representative 3D reconstruction images of P21 WT (left) and Pcdhg-cKO (right) symmetric (Sym.) (b) and asymmetric (Asym.) (c) clones labelled by MADM. The coloured lines indicate layer boundaries. For b and c, scale bars, 100 μm. d-g, Quantification of the pairwise (d,e) and maximal (f,g) lateral (d,f) and radial (e,g) distances between neurons in individual WT and Pcdhg-cKO clones. n = 158 clones, including 68 symmetric and 90 asymmetric clones, collected from around 480 brain sections from 8 brains (WT); and n = 164 clones, including 74 symmetric and 90 asymmetric clones, collected from around 475 brain sections from 8 brains (Pcdhg-cKO). Data are representative of four independent experiments. Statistical analysis was performed using two-tailed unpaired Student’s t-tests (d-g). The box plots are as described in Fig. 1.