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. 2023 May 25;16:1147597. doi: 10.3389/fnmol.2023.1147597

FIGURE 4.

FIGURE 4

Recombination levels do not correlate with behavior in p75Atoh1-CreERTM mice. Analysis of the recombination level of p75Atoh1-CreERTM adult mice after tamoxifen injection during development. (A,B) Immunostaining for Td-Tomato (red) and Dapi (blue) in p75Atoh1-CreERTM adult mouse injected with tamoxifen at embryonic day 17/18. (A) Expression of Td-Tomato in the cerebellum of two sibling mice that received the same tamoxifen doses. Note the high variability in the recombination levels. (B–G) Correlation analysis between the level of recombination and the behavioral results shown in this figure. The brains of all the mice injected with tamoxifen used in this figure were obtained, sectioned, and stained for Td-Tomato as a proxy of recombination level. (B) Correlation between the level of recombination and the time spent in the center of the open field arena. (C) Correlation between the level of recombination and the time spent in the open arm of the elevated zero maze. (D,E) Light-dark transition test: correlation between the level of recombination and (E) the time spent in the lit chamber or (D) the delay time to first cross to the illuminated chamber. (F,G) Correlation with the grooming test: correlation between the level of recombination and (F) the time the mice spent grooming or (G) the frequency of grooming.