Fig 3. Cleavage of substrate DNA by two different DNase II enzymes.

(A) The activities of bovine DNase II and TsDNase II-7 after coincubation of purified protein with substrate DNA were assayed using agarose gel electrophoresis (37°C, pH = 5, 1 h). (B) Equal amounts of different DNase II enzymes (2 μg of ExpiCHO-expressed DNase II protein samples, 100 units of commercialized bovine DNase II) and 2 μg of BSA were loaded on a 1% agarose gel (containing substrate DNA) and analyzed for DNase II activity under conditions known to activate DNase II activity, as described under the agar diffusion method (37°C, pH = 5, 1 h). 2 μg of BSA (negative control); 100 units of commercialized bovine DNase II and 2 μg of ExpiCHO-expressed bovine DNase II (positive control).