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. Author manuscript; available in PMC: 2023 Jun 8.
Published in final edited form as: Lab Chip. 2015 Dec 7;15(23):4423–4432. doi: 10.1039/c5lc01053e

Figure 2.

Figure 2

Desired assay temperature tolerances and power curves. A: Assay temperature specifications. The minimum assay temperature here is 47°C, and the temperature must warm up in 15 minutes or less, and hold for 25 minutes. To avoid permanently losing enzyme activity, this assay must never exceed 55°C. An example temperature profile is shown (gray line) that meets these specifications. B: Ideal power profiles to achieve isothermal heating for a range of ambient temperatures, along with an example power profile from a chemical power supply. Cold ambient conditions need more power to warm the system up, in addition to needing more power to account for steady-state heat losses. The non-ideal power profile requires that temperature be additionally regulated by other means (e.g., PCM).