Table 1.
List of compounds that exhibited the highest observed affinity for each CA isozyme as determined by TSA, selected from the entire list of compounds in the PLBD
| Isozyme | Compound | K d,obs (nM) | K d,int (nM) |
|---|---|---|---|
| CAI | VD11-61 | 0.025 | 0.00066 |
| CAII | VD10-49 | 0.79 | 0.045 |
| CAIII | TFMSA; TFS | 1000 | 220 |
| CAIV | EA3-2 | 1.4 | 0.0042 |
| CAVA | EZA | 19 | 1.7 |
| CAVB | VD12-05 | 0.050 | 0.0017 |
| CAVI | TFMSA; TFS | 14 | 1.2 |
| CAVII | VD10-49 | 0.22 | 0.011 |
| CAIX | VD11-4-2 | 0.083 | 0.00078 |
| CAXII | EA12-3 | 0.40 | 0.0015 |
| CAXIII | VD11-9 | 0.28 | 0.021 |
| CAXIV | VD10-49 | 0.50 | 0.025 |
The corresponding intrinsic affinities calculated as described in (17) are also listed for each compound–protein pair. All values are determined at 37°C.