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. 2000 Jan 15;28(2):634–640. doi: 10.1093/nar/28.2.634

Figure 2.

Figure 2

Figure 2

DNA-primed strand transfer. (A) Gel fractionation of the transfer products. Labeled ODN (40 nM) was heat annealed to donor RNA (100 nM) and incubated in the absence (lanes 1–3) or presence of 200 nM WT (lanes 4–14), S (lanes 5–15) or Δ (lanes 16–26) acceptor RNAs and 200 nM HIV-1 RT. Reaction was for 30, 36, 42, 48, 54, 60, 66, 72, 78, 84 or 90 min. (B) Quantification of the full-length transfer product obtained under the same conditions, except that the RT concentration was reduced to 100 nM.