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tRNA3Lys-primed strand transfer in the absence and presence of NCp. (A) Gel fractionation of the transfer products. tRNA3Lys (40 nM) was heat-annealed to 100 nM donor RNA and DNA synthesis was initiated by addition of 50 µM dNTP, 200 nM acceptor RNA, 100 nM RT and saturating amounts of NCp (1 NCp:7–8 nt). (B) Quantification of the gel shown in (A). Reaction time was 1 (gray) or 2 h (black).
