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. 2023 Apr 7;51(10):e56. doi: 10.1093/nar/gkad244

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Reproduction of major AISO patterns from Kim et al. (2017) using Insplico. (A) Schematic representation of the different groups of exons investigated for a representative gene. (B) Distribution of exons according to Fupfi values generated by Insplico for second-first (left), truly internal (middle) and second-last (right) exons as defined by extract_exons_from_gtf.pl. (C) Distribution of exons according to Fupfi values for second-first (left), truly internal (middle) and second-last (right) exons as provided by Kim et al. N(upfi + dofi) for internal and second-last exons was not provided in the original publication. (D) Distribution of second-first exons as annotated by Kim et al. according to exon types extracted by extract_exons_from_gtf.pl. ‘Diverse’ correspond to exons with more than one type of annotation across transcripts. (E) Distribution of exon types as extracted by extract_exons_from_gtf.pl for all exons in the Ensembl hg19 annotation. (F) Distribution of strictly defined second-first exons from Kim et al.’s original set according to Fupfi values as provided by Kim et al. (15).